30,000 mouse brain tissue cells were used in a standard 3'RNA single cell experiment.

The library were generated using MobiCube 3’RNA v2.0 kit and sequencing on illumina NovaSeq 6000 using paired end 150.

Analysis was followed by default parameters in MobiVision v2.1 and MobiVision v1.0, respectively. Intron was included in analysis, and EmptyDrops was used for cell-calling.

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